Publications

Journal covers

First authorships 

Nicolas, W.J., Fäßler, F., Dutka, P., Schur, F.K.M., Jensen, G., Meyerowitz, E., 2022. Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks. Current Biology 32, 2375-2389.e6 (

Nicolas WJ, Jensen G.J., Meyerowitz E, 2022. Cryo-electron tomography to study the structure of the primary cell wall in Allum cepa. Bio-protocol

William J. Nicolas, Debnath Ghosal, Elitza I. Tocheva, Elliot M. Meyerowitz, Grant J. Jensen. Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography. Journal of Bacteriology Jan 2021, 203 (3).

Nicolas WJ, Bayer EM, Brocard L. 2017. The Study of Three-dimensional Structure of Plasmodesmata in Plant Tissues by Electron Tomography. From High Pressure Freezing Preparation to EM Ultrathin Section on-grid Deposition. Bio-protocol 7.

Nicolas W, Grison MS, Trepout S, Gaston A, Fouché M, Cordelières FP, Oparka KJ, Tilsner J, Brocard L, Bayer EM. 2017. Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves. Nature Plants 3. 

Nicolas W, Grison MS, Bayer EM. 2018. Shaping plasmodesmata intercellular channels: The structure-to-function missing link. Journal of Experimental Botany 69.

Co-authorships 

Kaplan, M., Chang, Y.-W., Oikonomou, C.M., Nicolas, W.J., Jewett, A.I., Kreida, S., Dutka, P., Rettberg, L.A., Maggi, S., Jensen, G.J., 2023. Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus. Accepted at Nature microbiology

Kaplan, M., Chreifi, G., Metskas, L.A., Liedtke, J., Wood, C.R., Oikonomou, C.M., Nicolas, W.J., Subramanian, P., Zacharoff, L.A., Wang, Y., Chang, Y.-W., Beeby, M., Dobro, M., Zhu, Y., McBride, M., Briegel, A., Shaffer, C., Jensen, G.J., 2021. In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. eLife 10.

Cody Gillman, William J. Nicolas, Michael W. Martynowycz, Tamir Gonen. Design and implementation of suspended drop crystallization. Accepted at International Union of Crystallography journal 2023.03.28.534639; doi: https://doi.org/10.1101/2023.03.28.534639

Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T., 2023. A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals. Nature communications

Mohammed Kaplan, William J Nicolas, Wei Zhao, Stephen D carter, Lauren Ann Metskas, Georges Chreifi, Debnath Ghosal and Grant J Jensen. In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography. In: Gonen T., Nannenga B.L. (eds) cryoEM. Methods in Molecular Biology, vol 2215. Humana, New York, NY.

Petit, Jules D., Li, Ziqiang Patrick, Nicolas, William J., Grison, Magali S. & Bayer, Emmanuelle M. Dare to change, the dynamics behind plasmodesmata-mediated cell-to-cell communication. Current Opinion in Plant Biology vol. 53 80–89 (2020)

Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., … Bayer, E. M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports.

Yan, D., Yadav, S. R., Paterlini, A., Nicolas, W. J., Belevich, I., Grison, M. S., … Bayer, E. M. (2019). PLM modulates phloem unloading through sphingolipid biosynthesis and plasmodesmal ultrastructure. Nature Plants, 5(6), 604–615.

Tilsner J, Nicolas W, Rosado A, Bayer EM. 2016. Staying tight: plasmodesmal membrane contact sites and the control of cell-to-cell connectivity in plants. Annual Review of Plant Biology 67, 1–28.

Grison, M.S.a, Brocard, L.a, Fouillen, L., Nicolas, W., Wewer, V., Dörmann, P., Nacir, H., Benitez-Alfonso, Y., Claverol, S., Germain, V., et al. (2015). Specific membrane lipid composition is important for plasmodesmata function in Arabidopsis. The Plant Cell 27, 1228–1250;    aco-first authors